鸡白痢沙门菌噬菌体GSP040的生物学特性及基因组分析OA

A virulent phage against Salmonella Pullorum was isolated and purified from fecal samples of chicken farms and designated as GSP040.Transmission electron microscopy observation showed that the phage belonged to the Siphoviridae family in morphology,with virion characterized by a typical icosahedral head and a non-contractile tail.Host range analysis demonstrated that GSP040 could efficiently lyse Salmonella Pullorum,and also exhibited lytic activity against Salmonella Typhimurium and Salmonella Enteritidis.The optimal multiplicity of infection(MOI)was 0.1.The One-step growth curve analysis indicated that the latent period of 10 min and the burst period was 80 min.The phage possessed favorable environmental stability,maintaining high lytic activity at pH ranging from 3 to 11 and temperatures ranging from 4 ℃ to 50 ℃.Whole-genome sequencing results revealed that GSP040 was a double-stranded DNA virus with a full genome length of 42,621 bp and a GC content of 50％.A total of 69 predicted open reading frames(ORFs)were encoded,and no known virulence-related genes,drug resistance-related genes or lysogeny-related genes were detected.In conclusion,GSP040 is a virulent phage with a broad lytic spectrum,strong lytic capability,and excellent environmental adaptability,which has significant research value and application potential in the development of biological agents for the prevention and control of Salmonella infections caused by Salmonella Pullorum and other related strains.