海伦兜兰组培苗生根培养体系优化OA

Paphiopedilum helenae is national first-class key protected wild plant with extremely small popula-tion.Number of its individuals is far below minimum viable population threshold,and it is at risk of extinction at any time.In order to optimize rooting culture system for P.helenae tissue culture seedlings,effects of basic media,exogenous plant hormones and organic additives on rooting of tissue culture seedlings were analyzed,and the most suitable rooting medium was selected.Results showed that rooting effect of basic media was in or-der of 1/2MS>MS>Hyponex No.1.Exogenous plant hormones had concentration effect,and both 1.0 mg/L NAA and 0.5 mg/L IBA could effectively promote rooting,combination of which had significant additive effect.The best combination of organic additives was 10%coconut water+5%banana,followed by 15%coconut water and 10%coconut water+0.2%peptone,and addition of 10%banana alone was not conducive to rooting.Synthesiz-ing growth conditions of roots and plants,the most suitable rooting medium for P.helenae tissue culture seed-lings was 1/2MS+1.8 mg/L 6-BA+1.0 mg/L NAA+0.5 mg/L IBA+10%coconut water+5%banana.