铅镉胁迫对不同羊肚菌菌丝生长及富集响应的影响OA

[Objective]The effect of Cd and Pb stress on damage of different Morchella strains was investigated to provide a scientific basis for breeding Morchella strains with a higher tolerance to heavy metals stress.[Method]9 Morchella strains of Shangdong LM,Lefeng 13,DB33-5,and so on were cultured on media containing Pb and Cd with five different concentrations of 2 mg/L,4 mg/L,6 mg/L,8 mg/L and 10 mg/L to investigate the effects of Pb and Cd stress on growth rate,colonial morphology,antibacterial rate,biomass and heavy metal content of mycelia of different Morchella strains.[Result]The mycelium growth rate of 9 Morchella strains showed a general downward trend with increase of Pb and Cd concentration.When Pb concentration was 10 mg/L,the mycelium growth rate of Shangdong LM strain reached 7.35 mm/d,significantly higher than other strains.The mycelium growth rate of Shangdong LM and DB8-4 strain still kept more than 4.00 mm/d and 3.82 mm/d when Cd concentration was 10 mg/L respectively.With increase of Pb concentration,the colonial density decreased continuously,its aerial mycelia reduced,and the mycelium color of some Morchella strains became yellow.The mycelium of Lefeng 13 Morchella strain formed an irregular inhibition zone when Pb concentration was 6-10 mg/L.The colony of some Morchella strains stopped growing almost under high Cd concentration.With increase of Pb and Cd concentration,the mycelial biomass of 9 Morchella strains showed a declining trend overall.The mycelial biomass of 9 Morchella strains under Cd stress was lower than that under Pb stress generally.The Pb content in mycelial of 9 Morchella strains rose with increase of Pb concentration,and the Pb content in mycelial of DB29-2 strain reached 3 991.67 mg/kg(the highest)when Pb concentration was 10 mg/L.The Cd content in mycelial of 9 Morchella strains showed two different patterns with increase of Cd concentration.The Cd content in mycelial of Morchella strains belonging to yellow clade and black clade rose continuously and showed an increasing first and then decreasing trend with increase of Cd concentration respectively.The Cd content in mycelial of DB41-1 strain reached 11 194.02 mg/kg when Cd concentration was 10 mg/L.[Conclusion]The high Cd concentration has the stronger inhibitory effects on mycelium growth rate and biomass of 9 Morchella strains compared with those of high Pb concentration.The Cd enrichment capacity of 9 Morchella strains is higher than their Pb enrichment capacity.DB8-4 Morchella strain belonging to yellow clade has a higher tolerance to mycelial growth rate.Shangdong LM Morchella strain has the higher Cd enrichment capacity among 9 Morchella strains.