介质阻挡放电等离子体对消除灰葡萄孢菌的机理研究OA

In this study,the sterilization efficiency and underlying mechanism of dielectric barrier discharge(DBD)plasma against B.cinerea were systematically evaluated.The optimal treatment parameters were first determined based on the effects of treatment duration and voltage on inactivation efficiency.Subsequently,the fungicidal mechanism of DBD was investigated by analyzing changes in colony morphology,spore ultrastructure,and cell membrane integrity.The results showed that with a spore concentration of 2×10⁶ CFU/mL,an inactivation rate of up to 99.75%was achieved at 10 kV and 60 s.After DBD treatment,the colony diameter,sporulation quantity,mycelial biomass accumulation,and ergosterol content of B.cinerea were significantly reduced.Meanwhile,cell membrane permeability increased,accompanied by elevated protein and nucleic acid leakage,higher malondialdehyde(MDA)content,and enhanced propidium iodide(PI)fluorescence intensity.Reactive oxygen species(ROS)levels were markedly increased,whereas fluorescein diacetate(FDA)staining was completely lost.Observations via scanning electron microscopy(SEM)and transmission electron microscopy(TEM)further confirmed that DBD treatment caused spore membrane rupture,ultrastructural damage,and leakage of intracellular contents.Overall,DBD effectively inactivates B.cinerea through multiple damage pathways,including disruption of spore morphology,cell membrane integrity,and internal structures.