不同饼肥与施用量对烤烟土壤特性及细菌多样性的影响OA

[Objective]The effects of different cake fertilizers with different application rates on properties and bacterial communities of flue-cured tobacco planting soil were investigated to provide the scientific basis for selecting suitable fertilization schemes in cultivation of flue-cured tobacco.[Method]The effects of 3 fertilizers[bean cake fertilizer(A1),sesame cake fertilizer(A2),and rapeseed cake fertilizer(A3)]and tobacco specific fertilizer(CK)]with two application rates of 1 200 kg/hm2(B1)and 1 800 kg/hm2(B2)on nutrient content,enzyme activity and bacterial community distribution of flue-cured tobacco planting soil were analyzed by using a two-factor randomized block.[Result]Three cake fertilizers with application rate of 1 800 kg/hm2 all could improve physicochemical properties and carbon to nitrogen ratio of flue-cured tobacco planting soil and increase activity of soil enzymes.The dominant bacteria phyla were Proteobacteria and Actinobacteriota.The dominant bacteria genera were Sphingomonas and Subgroup_6.Applying sesame cake fertilizer could increase the relative abundance of bacterial communities in rhizosphere soil at budding stage of flue-cured tobacco plants at phylum and genus level.LEfSe and Metagenome Seq analysis showed that the main phyla affecting the difference in bacterial communities of rhizosphere soil were Proteobacteria and Actinomycetes.RDA analysis showed that the content of soil available potassium and organic matter was the key factor influencing the distribution of soil bacterial communities.[Conclusion]Applying sesame cake fertilizer with application rate of 1 800 kg/hm2 can improve soil nutrient status,soil enzyme activity and the relative abundance of bacterial communities in rhizosphere soil at phylum and genus level to promote soil sustainability in flue-cured tobacco growing area of Jinping Town,Jingdong County,Yunnan and other flue-cured tobacco growing area with the similar ecology condition.