伽马射线照射对小鼠肠道干细胞及分化细胞关键基因的影响及鉴定OA

This study aims to investigate the effect of gamma irradiation on the expression profile of intestinal stem cells(ISCs)and their differentiated cells in the intestine of mice,as well as to screen for key genes.The Seurat package was used to perform cluster analysis and differential expression analysis on a single-cell sequencing dataset(GSE165318)to identify differentially expressed genes in each cell type.The TCseq package analyzed the expression trend over time.Key genes were identified by intersecting up-regulated genes with specific trend gene clusters across the five cell types.The clusterProfiler package and STRING database were used for gene ontology(GO)enrichment analysis and PPI network analysis of key genes.A mouse model of radiation-induced intestinal injury(RIII)was established via abdominal irradiation with 12 Gy of 60Co γ-rays.Intestinal tissues were collected at 0,3,and 7 days post-irradiation in control and treatment groups.Key genes were verified using real-time quantitative PCR(RT-qPCR).The results showed that twelve intestinal cell types were identified,and the key gene Mmp8 was found in ISCs and their differentiated cells.Additionally,the expression of 15 Mmp family members peaked on the 3rd day after irradiation,with heterogeneous expression across different cell types.PPI network analysis revealed that Mmp3,Mmp7,Mmp8,Mmp9,and Mmp11 are central in the regulatory network.The differential expression of Mmp8,Mmp9,Mmp13,Mmp19,and Mmp25 was validated by RT-qPCR.The Mmp family plays an important role in the process of intestinal injury caused by gamma rays and its repair,and Mmp8,Mmp9,Mmp13,Mmp19 and Mmp25 may be potential therapeutic targets for intestinal radiation injury.