姬竹叶石斛叶绿体基因组特征分析OA

[Objective]The chloroplast genome characteristics of Dendrobium odiosum was investigated to provide a reference for botanic classification,genetic evolution,development and utilization of D.odiosum.[Method]The chloroplast genome sequence of D.odiosum obtained by high-throughput sequencing and third generation ONT sequencing technology was used to analyze the structure,characteristics and phyletic evolution relationship of the chloroplast genome in D.odiosum.[Result]The D.odiosum chloroplast genome with a typical tetrad structure and 157 312 bp of sequence length contained 116 unigenes,8 rRNAs,38 tRNAs and 70 protein-coding genes.63 detected SSR sites were mainly mono-and di-nucleotides,and the base compositions were mostly A and T repetitive units.There were 18 061 amino acids in cpDNA coding sequence of D.odiosum.The codons of CDS sequence preferred to end with A/U(T)bases.There was a close genetic relationship between D.odiosum,Dendrobium salaccense and Dendrobium loddigesii.There was a distant genetic relationship between D.odiosum and Dendrobium hancockii and a significant difference in the IR/SC boundary between D.odiosum and D.hancockii.[Conclusion]The chloroplast genome of D.odiosum has a typical tetrad structure,and there is a distant genetic relationship between D.odiosum and D.hancockii.It is speculated that D.odiosum is a separate species.