栽培温度对金线莲次生代谢产物的影响OA

[Objective]Temperature is a key environmental factor regulating secondary metabolism of Anoectochilus roxburghii.This study systematically investigated the effects of different cultivation temperatures on the content of secondary metabolites in A.roxburghii,providing a scientific basis for optimizing artificial cultivation conditions and improving the quality of medicinal materials.[Method]Using A.roxburghii from Meihuashan as the test material,plants were treated with four temperatures(5℃,15℃,25℃,35℃)for 7 days in an artificial climate chamber.Untargeted metabolomic analysis was performed using LC-MS/MS,combined with multivariate statistical methods,to systematically compare the differences in secondary metabolites,including lipids,carbohydrates and their derivatives,flavonoids and organic heterocyclic compounds,terpenoids,alkaloids and their derivatives.The contents of goodyeroside A and kinsenoside were determined by HPLC.[Result]Temperature treatments significantly altered the composition of secondary metabolites in A.roxburghii.Carbohydrates and their derivatives accumulated significantly at low temperatures(5℃,15℃),while their content dropped sharply to 1.2%of the 25℃treatment group at high temperatures(35℃).Flavonoids and organic heterocyclic compounds significantly increased under both high and low temperatures,showing characteristics of stress-induced induction.Terpenoid substances significantly increased at low temperatures and decreased at high temperatures,but its proportion in the composition of secondary metabolites remained unchanged;while alkaloids and their derivatives,lipids,and the medicinal components kinsenoside and goodyeroside A showed an optimal temperature accumulation pattern,with peak levels at 25℃.Specifically,the contents of goodyeroside A and kinsenoside reached 103.2 mg/g and 92.43 mg/g respectively,at 25℃,and decreased by 20%to 30%under high or low temperature treatments.[Conclusion]Temperature is the key environmental factor regulating the synthesis of secondary metabolites in A.roxburghii.Different secondary metabolites respond to temperature in diverse ways.A temperature of 25℃is most conducive to the growth of A.roxburghii and the accumulation of medicinal components such as alkaloids,goodyeroside A and kinsenoside,while antioxidant components such as flavonoids and organoheterocyclic compounds can be enriched through temperature stress induction.This study provides a metabolomic basis for the precise,goal-oriented cultivation management of A.roxburghii.