旋覆花中酚酸类化合物的降脂活性研究OA

Objective To isolate and identify phenolic acid constituents from methanol extract of Inula japonica Thunb.and evaluate their lipid-lowering activity in vitro.Methods The methanol extract of Inula japonica Thunb.was purified using silica gel,ODS,and semi-preparative HPLC.The compound structures were determined using physicochemical and spectroscopic analyses,and their hypolipidemic activities were assessed in an AML12 cell model of free fatty acids(FFA)-induced lipid deposition.The active compounds were further assessed for their effects on lipid deposition,reactive oxygen species(ROS)production and mitochondrial membrane potential(MMP)of the cell model.Results A total of 11 phenolic acid compounds were isolated from Inula japonica Thunb.,namely dihydrocaffeic acid(1),protocatechualdehyde(2),chlorogenic acid(3),cryptochlorogenic acid(4),1,3-O-dicaffeoylquinic acid(5),2,3-dicarboxy-6,7-hydroxy-l-(3',4'-dihydroxy)-phenyl-1,2-dihydronaphthalene-10-methyl ester(6),caffeic acid(7),protocatechuic acid(8),3-carboxy-6,7-dihydroxy-1-(3',4'-dihydroxyphenyl)-naphthalene(9),p-hydroxybenzoic acid(10),and 4-hydroxyphenylacetic acid(11).Among them,compounds 1 and 9 were isolated for the first time from the genus Inula,and compound 6 was isolated for the first time from this plant.Compounds 6 and 9 showed lipid-lowering effects in FFA-induced AML12 cells,significantly lowered cellular levels of total cholesterol,triglyceride,alanine aminotransferase,and aspartate aminotransferase,reduced lipid accumulation,and alleviated oxidative stress and mitochondrial function.Conclusion Inula japonica Thunb.contains diverse phenolic acids,and among them compounds 6 and 9 show hypolipidemic activities via inhibiting lipid accumulation and improving oxidative stress and mitochondrial function and can potentially serve as lead compounds for treatment of metabolic dysfunction-associated steatotic liver disease.