杨芽黄素对胰腺癌细胞PANC-1迁移和铁死亡的影响OA

Investigated the impact of TEC on the migration and ferroptosis of pancreatic cancer cells PANC-1.The toxicity of TEC on PANC-1 cells was measured by MTT assay,the data were analyzed and grouped.The effects of TEC on cell proliferation and migration was evaluated by the colony formation and Transwell assay.Kits and flow cytometry were used to observe assess the changes of glutathione(GSH),malondialdehyde(MDA),ferrous ion and lipid reactive oxygen species(Lipid ROS)levels in cells.The protein expression of glutathione peroxidase 4(GPX4),Ferritin heavy chain(FTH1),acyl-CoA synthetase long-chain family member 4(ACSL4)were evaluated by Western Blot.Results shows that with increasing TEC concentration,PANC-1 cell viability,colony formation,migration ability,GSH concentration,GPX4 and FTH1 decreased;while MDA,Fe2+concentration,lipid ROS levels,and ACSL4 protein increased.The ferroptosis inhibitor Ferrostatin-1 restored TEC-induced cell death and migration ability,and restored the expression of GPX4,FTH1,and ACSL4 proteins.These results suggest that TEC can induce ferroptosis in PANC-1 cells and inhibit cell migration.