紫草素诱导肝癌细胞凋亡和自噬效应及其对ROS水平的影响OA

Objective To explore the mechanism of action of purpurin in inhibiting liver cancer cell line SMMC-7721.Methods SMMC-7721 cells were cultured in vitro and treated with different concentrations of shikonin.The inhibitory rate of relative cell viability was as-sessed using the MTT assay after 72 hours;cell survival rate was evaluated via trypan blue exclusion assay after 24 hours;flow cytometry was employed to analyze apoptosis rate,ROS levels,autophagy activity,and cell cycle distribution in groups treated with different concen-trations of shikonin.Results Shikonin inhibited relative cell viability in a concentration-dependent manner(IC₅₀=1.25 mg/L).Com-pared with the control group,the early apoptosis rate significantly increased in the 0.25 mg/L group(26.89%vs.1.87%,P<0.05);the mean autophagy fluorescence intensities in the 1 and 4 concentration groups were 12.63%and 11.78%,respectively(control group:7.96%,P<0.05).The proportion of viable cells in the 4 concentration group decreased from 95.59%to 42.45%.ROS levels significantly increased in the 1 mg/L and 4 mg/L groups(P<0.05).Treatment with 4 mg/L shikonin increased the proportion of SubG ₁ phase cells(52.22%)and decreased the proportions of S phase and G ₂/M phase cells(P<0.01).Conclusion Shikonin can inhibit the relative vi-ability of hepatocellular carcinoma cells and induce apoptosis and autophagy,effects that may be related to increased ROS levels.