大豆GmAP2-35两种可变剪切转录本的盐碱胁迫功能差异及机制OA

Soybean(Glycine max)is an important food and oil crop,and its yield and quality are often severely restricted by salt-alkali stress.To determine whether alternative splicing of the GmAP2-35 member of the soybean AP2 transcription factor family leads to differences in salt-alkali stress responses,two splicing transcripts(GmAP2-35-1 and GmAP2-35-2)were identified and their functions under salt-alkali stress were compared.By constructing overexpression vectors of the two transcripts and obtaining transgenic soybean lines,they were subjected to salt-alkali treatment,and phenotypes were observed,growth indicators were statistically analyzed,and antioxidant-related physiological and biochemical indicators were measured to analyze the response differences of the different transcripts.The results showed that GmAP2-35-2 was significantly induced under salt-alkali stress,while the expression level of GmAP2-35-1 did not change significantly;the transgenic lines overexpressing GmAP2-35-2 maintained a better growth state under salt-alkali stress,with higher plant height and fresh weight than the GmAP2-35-1 lines,and their SOD(superoxide dismutase),POD(peroxidase),and CAT(catalase)activities were significantly enhanced,while MDA(malondialdehyde)content was significantly reduced and ROS(Reactive Oxygen Species)accumulation was less.The results indicated that alternative splicing of GmAP2-35 led to functional differentiation in salt-alkali stress responses,with GmAP2-35-2 playing a positive regulatory role in enhancing soybean salt-alkali stress tolerance.This study revealed the mechanism by which alternative splicing of AP2 transcription factors functions in soybean's tolerance to adverse conditions,providing new molecular evidence for a deeper understanding of how soybean finely regulates its response to salt-alkali stress through alternative splicing,and offering new research targets for conducting salt-alkali-tolerant molecular breeding using specific transcripts.