重组胶原蛋白伤口愈合水凝胶的制备及性能OA

Recombinant collagen hydrogels are ideal materials for promoting wound healing but currently suffer from weak mechanical strength and single functionality,which limit their practical applications.A multifunctional hydrogel with a multi-network structure polymethylacrylamide-acrylic acid/tannic acid/recombinant collagen(MA-AAC/TA/COL)is prepared using in-situ polymerization.The main raw materials include recombinant collagen,tannic acid,methylacrylamide,and acrylic acid.The microstructure,mechanical properties,swelling performance,adhesive property,self-healing ability,antioxidant activity,antibacterial performance,and biocompatibility of hydrogels with different components are explored.The results show that the multi-network structure design effectively enhances the mechanical properties of the hydrogel.Its maximum tensile strain reaches(250.2±2.7)%and maximum tensile stress is(130±3)kPa.It can withstand 80%compressive deformation without breaking and the maximum compressive strength is(4.23±0.18)MPa.The hydrogel exhibits certain pH sensitivity with different swelling rates under various pH conditions.It has a maximum adhesive strength of(36.27±5.03)kPa on pig skin,a self-healing rate of 67.7%and an in-vitro antioxidant rate of(80.2±0.9)%.It shows good antibacterial effects against E.coli and S.aureus with an antibacterial rate close to 99.99%.Additionally,the hydrogel has low cytotoxicity with a cell survival rate greater than 80%.The MA-AAC/TA/COL hydrogel is expected to serve as a multifunctional wound dressing and find application in the field of wound treatment.