生长年限对蒙古黄芪根际土壤环境的影响OA

Taking Astragalus membranaceus(Fisch.)Bge.var.Mongholicus(Bge.)Hsiao(A.mongholicus)seeds and seedlings as test materials,the effects of growth years on the bacterial community diversity,metabolites,and enzyme activity of A.mongholicus rhizosphere soil were studied by using 16S rRNA microbial diversity sequencing and LC-MS metabolomics,in order to provide reference for the ecological cultivation of A.mongholicus.The results showed that,1)Alpha diversity analysis revealed that with the increase of growth years,the Observed species index and Chao1 index of the rhizosphere soil bacterial community of A.mongholicus showed a trend of first decreasing and then increasing,while the Shannon index and Simpson index showed little change trend.Beta diversity analysis revealed that at the genus level,there were 47,26,and 12 differential species in H1-vs-HCK,H2-vs-HCK,and H1-vs-H2.The predicted functional genes were enriched in KEGG pathways such as carotenoid biosynthesis,glycerolipid metabolism,and glycerophoripip metabolism,etc,respectively.2)Metabolomics analysis revealed there were 538,225,and 559 differential metabolites in H1-vs-HCK,H2-vs-HCK,and H1-vs-H2.These metabolites were enriched in 128,70,and 150 KEGG pathways.Correlation analysis revealed that the certain differential species relative abundance were significantly correlated with the differential metabolites expression levels.3)Enzyme activity analysis revealed that increased growth years reduced the catalase,alkaline phosphatase,and urease activities in the rhizosphere soil,while increasing the sucrase activity.Correlation analysis found a highly significant correlation between the bacterial community diversity and the certain enzymes activity.In summary,A.mongholicus seeds and seedlings have different effects on the bacterial community diversity,metabolites,and enzyme activity of rhizosphere soil during their growth and development.