基于多重指标的不同黑木耳菌株营养生长特性评价OA

Taking 37 cultivated Auricularia heimuer strains as test materials,aniline blue plate decolorization tests,guaiacol plate chromogenic tests,antagonism tests,culture at different temperatures(15 ℃,20 ℃,and 25 ℃),and submerged fermentation combined with determination of extracellular and intracellular polysaccharide content and mycelial dry matter content were employed to investigate lignin-degrading enzyme production capacity,genetic differences,mycelial growth rate,extracellular and intracellular polysaccharide yield,and mycelial biomass of different strains,in order to provide reference for Auricularia heimuer depth development and utilization.The results showed that 23 strains with strong enzyme-producing ability were obtained through aniline blue and guaiacol plate screening;antagonism tests further analyzed the genetic diversity of these 23 strains.Mycelial growth rate increased significantly with temperature,with AH33('Ju 3')exhibiting the fastest growth rate at 20 ℃ and 25 ℃.Regarding polysaccharide yield,strain AH17('Xinke 10')had the highest extracellular polysaccharide yield(27.316 mg·mL-1),while AH22('Mudan 1')had the highest intracellular polysaccharide yield(60.193 mg·mL-1).Correlation analysis showed that fermentation mycelial biomass was extremely significantly positively correlated with mycelial growth rate at 15 ℃,but negatively correlated with growth rates at 20 ℃ and 25 ℃,as well as with intracellular and extracellular polysaccharide content.Comprehensive analysis indicated that strain AH17('Xinke 10')exhibited excellent performance in growth adaptability,polysaccharide yield,and biomass,making it the most promising candidate strain and recommended for subsequent high-yield cultivation or fermentation production.