基于Box-Behnken法优化宣木瓜幼果中酚酸及黄酮类7种成分的提取工艺OA

Objective To deeply optimize the extraction process for seven phenolic acids and flavonoids from young fruits of Chaenomeles speciosa Nakai.Methods With the young fruits of Chaenomeles speciosa Nakai as the research object,high-performance liquid chromatog-raphy was used for simultaneous determination of shikimic acid,malic acid,gallic acid,chlorogenic acid,proanthocyanidin B1,proanthocya-nidin B2,and catechin.Key influencing factors were screened based on single-factor experiments,and the Box-Behnken response surface design was used to optimize extraction process parameters with the total content of the seven components as the assessment index.Results The optimal extraction process conditions obtained were 0.3％phosphoric acid solution as the extraction solvent,a solid-liquid ra-tio of 1∶30 g/mL,and an extraction time of 30 minutes.Five validation experiments were conducted under these conditions,and the actual total content of the seven components was 47.136 mg,which was close to the predictive value of 46.707 mg.Extraction time had the grea-test influence on total extraction rate,followed by solid-liquid ratio and extraction solvent.Conclusion The model established has good predictability,and the optimized extraction process for young fruits of Chaenomeles speciosa Nakai is stable and feasible.