地构叶治疗类风湿关节炎的潜在活性物质研究OA

Objective To investigate the potential active substances in Speranskia tuberculata for the treatment of rheumatoid arthritis and their possible mechanisms.Methods A mouse model of rheumatoid arthritis was established by subcutaneous in-jection of a mixture of bovine type Ⅱ collagen and complete Freund's adjuvant,and the mice were randomly divided into blank group,model group.Tripterygium glycosides tablet group,high-/low-dose Speranskia tuberculata-petroleum ether site groups,high-/low-dose Speranskia tuberculata-ethyl acetate site(ST-EAC)groups,high-/low-dose Speranskia tuberculata-n-butanol site groups,and high-/low-dose Speranskia tuberculata-water site(ST-W)groups.The effect of Speranskia tubercu-lata extracts on rheumatoid arthritis was observed.The method of network pharmacology was used to obtain the potential ac-tive components of Speranskia tuberculata and their targets,and molecular docking was also performed to simulate the docking patterns between core targets and components and reveal the mechanism of action of Speranskia tuberculata.Results The high-and low-dose ST-EAC and the high-and low-dose ST-W significantly reduced arthritis scores,inhibited synovial hyper-plasia,alleviated inflammatory cell infiltration,and reduced the serum levels of interleukin-6,interleukin-1β,and tumor necro-sis factor-α.The active components of Speranskia tuberculata,such as aesculetin and apigenin-7-O-glucoside,might act on the key targets such as TNF,TP53,and AKT1,thereby affecting the important pathways in the pathogenesis of rheumatoid ar-thritis including endocrine resistance,MAPK,and prostaglandins in cancer.Conclusion The extract of Speranskia tubercula-ta can significantly improve collagen-induced rheumatoid arthritis,and its active substances aesculetin and apigenin-7-O-gluco-side can exert a therapeutic effect through multiple targets and pathways.