不同生长年限兰州百合根际土壤理化特性和微生物群落结构分析OA

[Objective]To explore the physicochemical characteristics and bacterial community structure of the rhizosphere soil of Lanzhou lilies with different growth years,and provide theoretical basis for the construction and maintenance of the rhizosphere soil microenvironment of Lanzhou lilies.[Method]This study focused on the rhizosphere soil of Lanzhou lilies with growth years of 4(G4),6(G6),and 8(G8)years,and measured their soil physicochemical indicators.At the same time,high-throughput sequencing technology was used to analyze the charac-teristics of soil bacterial communities.[Result]The results showed that as the growth years of Lanzhou lily increased,the pH value of the rhi-zosphere soil gradually decreased,and the organic matter and alkaline nitrogen showed a U-shaped change.The available phosphorus and a-vailable potassium first increased and then decreased.The content of nitrate reductase,urease,β-glucosidase and catalase showed a U-shaped change overall,but both were significantly higher than those in the CK group soil(P<0.05).The content of alkaline phosphatase increased with the growth years of Lanzhou lily.The content of polyphenol oxidase in G8 soil was significantly higher than that in G4 and G6(P<0.05).The results of bacterial diversity showed that the dominant phyla of Lanzhou lily soil with different growth years were Proteobacteria,Acidobac-teriota,Bacteroidota,Actinobacteriota,and Chloroflexi,with relative abundance exceeding 88%.The relative abundance of Proteobacteria and Acidobacteria was the highest,and there was no significant change between different growth years(P>0.05).The abundance of Bacteroi-dota and Acidobacteriota was the highest in G4 soil at 16.02%,while in G6 and G8 it was 13.10%and 14.96%,respectively,significantly higher than the CK group's 6.29%.The highest abundance of Acidobacteriota was 12.43%in G6,10.10%in G4,and decreased to 8.16%in G8,with no significant difference compared to the CK group.[Conclusion]In summary,the physicochemical and bacterial structure of the rhizosphere soil of Lanzhou lilies vary significantly with the growth years of Lanzhou lily.The regulation of factors such as soil pH,alkaline phosphatase,polyphenol oxidase,Bacteroidota,and Acidobacteriota with significant changes is beneficial for maintaining the healthy and sta-ble rhizosphere soil microenvironment of Lanzhou lilies.This result may provide a theoretical basis for achieving high-quality and high-yield cultivation of Lanzhou lilies.