多壁碳纳米管对水蕨生理特性的影响OA
Influence of Multi-walled Carbon Nanotubes on the Physiological Characteristics of Ceratopteris thalictroides(L.)Brongn.
在前期研究的基础上,以蕨类模式植物水蕨(Ceratopteris thalictroides)为供试材料,探究不同浓度多壁碳纳米管(MWCNTs)对水蕨生理特性的影响.结果表明,与对照组(0.0 mg/L MWCNTs)相比,T1(0.5 mg/L MWCNTs)、T2(1.0 mg/L MWCNTs)、T3(2.5 mg/L MWC-NTs)处理组对水蕨叶片中SOD活性有极显著影响(P<0.01),T4(5.0 mg/L MWCNTs)处理组对叶片中SOD活性有显著影响(P<0.05),其中,T2处理组叶片中SOD活性最低(636.18 U/g),T4处理组叶片中POD活性最低(319.06 U/g);MWCNTs处理对叶片中MDA和可溶性糖含量的影响均不显著(P>0.05),二者随MWCNTs浓度升高呈波动变化的趋势.水蕨DAB显色均呈现蓝紫色.综合研究结果显示,0.5~5.0 mg/L多壁碳纳米管处理可以影响水蕨孢子体的生理特性,在应对多壁碳纳米管处理时,SOD起主要作用;在高浓度处理下,SOD、POD活性显著变化,MDA含量升至最高,出现"高浓度抑制"现象.
Based on previous studies,this research used the fern model plant Ceratopteris thalictroides as test material to investigate the effects of different concentrations of multi-walled carbon nanotubes(MWCNTs)on the physiological characteristics.The results showed that compared with the control group(0.0 mg/L MWCNTs),the T1(0.5 mg/L MWCNTs),T2(1.0 mg/L MWCNTs),and T3(2.5 mg/L MWCNTs)treat-ments had extremely significant(P<0.01)effects on leaf SOD activity,while the T4(5.0 mg/L MWCNTs)treatment had a significant(P<0.05)effect.Among them,SOD activity was the lowest in the T2 group(636.18 U/g),and POD activity was the lowest in T4 group(319.06 U/g);MWCNTs treatment had no significant effect(P>0.05)on the contents of MDA and soluble sugar,both showed a fluctuating trend with the concentration of MWCNTs increased.DAB staining resulted in a blue-purple color.The comprehensive results indicate that treat-ments with 0.5-5.0 mg/L MWCNTs affect the physiological characteristics of C.thalictroides sporophytes,and SOD played a major role when exposed to the treatment of multi-walled carbon nanotubes;under high concentration treatment,the activities of SOD and POD changed signifi-cantly,and MDA content rose to the highest level,resulting in the"high concentration inhibition"phenomenon.
郭严冬;付娇娇;曹建国;郑玉红
江苏省中国科学院植物研究所(南京中山植物园)江苏省种质资源保护与利用重点实验室,江苏 南京 210014江苏省中国科学院植物研究所(南京中山植物园)江苏省种质资源保护与利用重点实验室,江苏 南京 210014上海师范大学生命科学学院,上海 200234江苏省中国科学院植物研究所(南京中山植物园)江苏省种质资源保护与利用重点实验室,江苏 南京 210014
农业科技
水蕨多壁碳纳米管孢子体生理特性
Ceratopteris thalictroides(L.)Brongn.Multi-walled carbon nanotubesSporophytePhysiological characteristics
《安徽农业科学》 2026 (10)
1-4,4
国家自然科学基金项目(30970267).
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