The diagnostic value of Xpert detection combined with nanopore sequencing for tuberculosis in HIV/AIDS patientsOA
Background:Tuberculosis(TB)remains a major cause of death in HIV/AIDS patients,where diagnosis is compli-cated by atypical presentation and paucibacillary disease.Current diagnostic methods have limitations in speed or sensitivity.This study evaluates the diagnostic value of rapid,portable nanopore sequencing for TB in this high-risk population.Methods:Clinical data and diagnostic results were collected from 59 HIV/AIDS patients with suspected tuber-culosis.The diagnostic performance of nanopore sequencing was compared with AFB staining,TB-DNA testing,Xpert,and solid mycobacterial culture,including sensitivity,specificity,positive predictive value(PPV),negative predictive value(NPV),Kappa coefficient,and the area under the receiver operating characteristic curve(AUC).Additionally,this study further evaluated the diagnostic performance of nanopore sequencing in combination with AFB staining,TB-DNA testing,Xpert,and culture methods.Results:Nanopore sequencing showed the highest performance among all methods,with a sensitivity of 60.50%,specificity of 90.50%,PPV of 92.00%,NPV of 55.90%,and AUC of 0.76.When used in combination with other methods,nanopore sequencing and Xpert achieved the best diagnostic performance,with a sensitivity of 71.10%,specificity of 85.70%,PPV of 90.00%,NPV of 62.10%,Kappa value of 0.523,and an AUC of 0.78.Conclusions:The combination of nanopore sequencing and Xpert demonstrates excellent diagnostic performance for diagnosing TB in HIV/AIDS patients.
Chang Song;Chunyan Zhao;Dan Luo;Aichun Huang;Chaoyan Xu;Jieqing Zhong;Yujie Mo;Zhentao Huang;Xiaoshi Lin;Zhouhua Xie;Qingdong Zhu
Department of Tuberculosis,The Fourth People’s Hospital of Nanning,Nanning 530023,China Clinical medical school,Guangxi Medical University,Nanning 530021,ChinaDepartment of Tuberculosis,The Fourth People’s Hospital of Nanning,Nanning 530023,China Clinical medical school,Guangxi Medical University,Nanning 530021,ChinaDepartment of Biostatistics,School of Public Health and Management,Guangxi University of Chinese Medicine,Nanning 530001,ChinaDepartment of Tuberculosis,The Fourth People’s Hospital of Nanning,Nanning 530023,ChinaDepartment of Biostatistics,School of Public Health and Management,Guangxi University of Chinese Medicine,Nanning 530001,ChinaDepartment of Biostatistics,School of Public Health and Management,Guangxi University of Chinese Medicine,Nanning 530001,ChinaDepartment of Biostatistics,School of Public Health and Management,Guangxi University of Chinese Medicine,Nanning 530001,ChinaClinical medical school,Guangxi Medical University,Nanning 530021,ChinaClinical medical school,Guangxi Medical University,Nanning 530021,ChinaDepartment of Tuberculosis,The Fourth People’s Hospital of Nanning,Nanning 530023,ChinaDepartment of Tuberculosis,The Fourth People’s Hospital of Nanning,Nanning 530023,China
医药卫生
Nanopore sequencingHIV/AIDSTuberculosisCombined diagnosis
《Infectious Medicine》 2026 (1)
P.1-8,8
funded by the Guangxi Zhuang Autonomous Region Health Commission Self-financed Scientific Research Project,grant numbers Z-A20231211 and Z20210352.
评论