Antimicrobial resistance and virulence patterns of Helicobacter pylori among patients with suspected peptic ulcer disease in disenfranchised settingsOA
Background:The epidemiology of Helicobacter pylori in Lebanon is underreported.We aimed to estimate the infec-tion proportion,clarithromycin resistance,and virulence determinants of H.pylori among symptomatic patients with suspected peptic ulcers in Tripoli,North Lebanon.Methods:Eighty-seven patients undergoing routine endoscopy screening for peptic ulcers were selected.Culture and Allplex^(TM)H.pylori and ClariR Assay were performed.Antimicrobial susceptibility testing for clarithromycin,tetracycline,levofloxacin,and rifampicin was performed using the E-test method.The virulence factors(cagA,vacA,dupA,iceA1,iceA2,and oipA)were screened and/or typed by PCR.Results:Overall,32.2%(28/87)of the patients were found to be infected with H.pylori.Five isolates were resistant to clarithromycin,associated with specific point mutations of A2143G(4/5)or A2142C(1/5).The vacA was found in all tested isolates,with s2m2 being the predominant vacA genotype.The 3′end variable region of cagA was identified in 44.4%of isolates,where cagA typing indicated their belonging to Western types.The other virulence determinants were dupA(77.8%),iceA1(61.1%),oipA(55.6%),and iceA2(50%).Conclusions:Our findings demonstrate the circulation of H.pylori among symptomatic patients with suspected peptic ulcers in Lebanon,characterized by a rich and diverse pool of virulence genes and the emergence of resistance to clarithromycin and levofloxacin.Large-scale surveillance is crucial for quantifying the burden of H.pylori infection,monitoring antimicrobial resistance trends,and ensuring timely updates to eradication treatment policies.
Marwan Osman;Rayane Rafei;Kevin J.Cummings;Wassim Rafei;Joseph Awada;Hayssam Moubayed;Farah Majzoub;Hassan Mallat;Fouad Dabboussi;Monzer Hamze
Department of Neurosurgery,Yale University School of Medicine,New Haven,CT 06510,USALaboratoire Microbiologie Santéet Environnement(LMSE),Doctoral School of Sciences and Technology,Faculty of Public Health,Lebanese University,Tripoli 1300,LebanonDepartment of Public and Ecosystem Health,College of Veterinary Medicine,Cornell University,Ithaca,NY 14853,USADivision of Digestive Surgery,Centre Hospitalier de Montélimar,Montélimar 26200,France Division of Digestive Surgery,Dar Al Chifaa Hospital,Tripoli 1300,Lebanon Division of Digestive Surgery,Monla Hospital,Tripoli 1300,LebanonDivision of Digestive Surgery,Haykel Hospital,Tripoli 1300,LebanonDivision of Digestive Surgery,Nini Hospital,Tripoli 1300,Lebanon Division of Digestive Surgery,Clinique de La Source,Lausanne 1004,SwitzerlandDivision of Digestive Surgery,Nini Hospital,Tripoli 1300,LebanonLaboratoire Microbiologie Santéet Environnement(LMSE),Doctoral School of Sciences and Technology,Faculty of Public Health,Lebanese University,Tripoli 1300,LebanonLaboratoire Microbiologie Santéet Environnement(LMSE),Doctoral School of Sciences and Technology,Faculty of Public Health,Lebanese University,Tripoli 1300,LebanonLaboratoire Microbiologie Santéet Environnement(LMSE),Doctoral School of Sciences and Technology,Faculty of Public Health,Lebanese University,Tripoli 1300,Lebanon Clinical Laboratory,Microbiology Department,Nini Hospital,Tripoli 1300,Lebanon
医药卫生
Helicobacter pyloriAntimicrobial resistanceMolecular methodAllplex^(TM)H.pylori and ClariR assayVirulence factorLebanon
《Infectious Medicine》 2026 (1)
P.87-91,5
supported by a grant from the Lebanese National Council for Scientific Research(CNRS-L Grant Research Program-2017)and the Lebanese University.
评论