Huangqin decoction ameliorates ulcerative colitis by regulating ferroptosis based on the integrative analysis of serum pharmaco-chemistry with metabolomics and network pharmacologyOA
Background:Huangqin decoction(HQD),a classic formula for treating ulcerative colitis(UC),exhibits anti-inflammatory and intestinal mucosa protective effects.However,its mechanisms require further investigation.This study aimed to elucidate these mechanisms by integrating metabolomics and serum pharmacochemistry-based network pharmacology.Methods:A dextran sulfate sodium(DSS)-induced mice model of UC was established to assess the therapeutic effect of HQD.Chemical compounds and absorbed constituents of HQD were identified by UHPLC-Q-Orbitrap-MS.Network pharmacology predicted targets and pathways based on blood-absorbed constituents.Differential metabolites and associated pathways were identified by serum metabolomics.Fe2+and GSH levels in colon tissues were measured to assess ferroptosis.Molecular docking evaluated binding affinities,and the expression of ferroptosis-related targets was validated by RT-qPCR and Western blot.Results:A total of 92 chemical compounds and 66 blood-absorbed constituents were identified.Compared to the DSS group,44 differential metabolites were reversed in the HQD group,enriched in tryptophan metabolism,arginine and proline metabolism,and pyrimidine metabolism.Joint analysis of network pharmacology and metabolomics focused on the arachidonic acid pathway and ferroptosis-related targets.HQD inhibited ferroptosis,evidenced by decreased Fe2+levels and restored GSH content.RT-qPCR,Western blotting,and molecular docking demonstrated that HQD bioactive components exhibited significant binding affinity for and regulatory activity toward four key ferroptosis-related targets:PTGS2,ALOX5,GPX4,and STAT3.Conclusion:The integrated analysis strategy suggests that the efficacy of HQD against UC may be associated with its regulation of inflammatory responses and ferroptosis-related targets,providing a preliminary basis for elucidating the material basis and mechanisms of HQD.
Jia-Yao Xiong;Xin-Yue Ji;Chong-Bo Zhao;Liu Yang;Jia-Hui Zheng;Ya-Jun Shi;Jing Wang;Min Wang
Shaanxi Key Laboratory of Traditional Chinese Medicine Foundation and New Drug Research,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaDepartment of Pharmacy,Nanjing Drum Tower Hospital,Nanjing University of Chinese Medicine,Nanjing 210008,ChinaShaanxi Provincial Engineering Technology Research Center for Traditional Chinese Medicine Decoction Pieces,Shaanxi University of Chinese Medicine,Xi’an 712046,China Shaanxi Traditional Chinese Medicine Processing Technology Heritage Base,Shaanxi University of Chinese Medicine,Xianyang 712046,ChinaShaanxi Key Laboratory of Traditional Chinese Medicine Foundation and New Drug Research,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaShaanxi Key Laboratory of Traditional Chinese Medicine Foundation and New Drug Research,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaShaanxi Key Laboratory of Traditional Chinese Medicine Foundation and New Drug Research,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaShaanxi Key Laboratory of Traditional Chinese Medicine Foundation and New Drug Research,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaDepartment of Pharmacy,Nanjing Drum Tower Hospital,Nanjing University of Chinese Medicine,Nanjing 210008,China
医药卫生
Huangqin decoctionulcerative colitisserum pharmacochemistrymetabolomicsferroptosis
《Traditional Medicine Research》 2026 (10)
P.20-33,14
financially supported by the National Natural Science Foundation of China(Grant No.81903892)the Natural Science Basic Research Program of Science and Technology Department of Shaanxi Province(Grant No.2025JC-YBMS-913)the Key Research and Development Program of Shaanxi Province(Grant No.2024SF-ZDCYL-03-11)the Shaanxi University of Traditional Chinese Medicine Project(Grant No.2023GP25)the Fourchain Integration Project of Qin Chuangyuan Industrial Innovation Gathering Area(Grant No.2024CY-JJQ-36).
评论