Exploring the targets and mechanism of Qianggu Ning in the treatment of postmenopausal osteoporosisOA
Background:Clinical observations have indicated that Qianggu Ning(QGN)can enhance bone density in postmenopausal women.We aimed to identify the primary active ingredients and targets of via network pharmacology and biological validation while exploring its potential mechanisms of action.Methods:We employed ultra-high-performance liquid chromatography-mass spectrometry to detect and analyze the active components of the QGN decoction.Topological analysis and target prediction were conducted in conjunction with network pharmacology.The binding efficiency of target proteins and small molecular compounds was verified using molecular docking technology.We simulated a mice model of postmenopausal osteoporosis(PMOP)through bilateral bilateral ovariectomy and assessed bone density changes in ovariectomized(OVX)mice after QGN intervention.We validated biological functions and target genes via pathological testing and quantitative polymerase chain reaction.Results:QGN effectively mitigates bone loss and lipid metabolism disorders in OVX mice.Using network pharmacology,we predicted that the key target genes for alleviating PMOP with QGN.The principal components of QGN underwent molecular docking with these five target genes,all demonstrating robust binding activity.Conclusion:This study confirms the efficacy of QGN in treating PMOP.QGN may achieve the desired effect of increasing bone density via pathways related to lipid,atherosclerosis and MAPK signaling.
Ning Li;Miao Luo;Huan Pei;De-Guang Li;Li Deng;Chong-Yu Zhao;Lei Li;Yuan Ma;Lv-Yu Li
Orthopedics Department,Kunming Municipal Hospital of Traditional Chinese Medicine,the Third Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650500,China Yunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment,Yunnan University of Chinese Medicine,Kunming 650500,ChinaYunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment,Yunnan University of Chinese Medicine,Kunming 650500,China School of Basic Medical Sciences,Yunnan University of Chinese Medicine,Kunming 650500,ChinaSchool of Integrative Chinese and Western Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,ChinaOrthopedics Department,Kunming Municipal Hospital of Traditional Chinese Medicine,the Third Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650500,China Yunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment,Yunnan University of Chinese Medicine,Kunming 650500,ChinaOrthopedics Department,Kunming Municipal Hospital of Traditional Chinese Medicine,the Third Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650500,ChinaOrthopedics Department,Kunming Municipal Hospital of Traditional Chinese Medicine,the Third Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650500,ChinaYunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment,Yunnan University of Chinese Medicine,Kunming 650500,China School of Basic Medical Sciences,Yunnan University of Chinese Medicine,Kunming 650500,ChinaOrthopedics Department,The Second Affiliated Hospital of Dali University,Dali University,Dali 671003,China Orthopedics Department,The Third People’s Hospital of Yunnan Province,Kunming 653100,ChinaOrthopedics Department,Kunming Municipal Hospital of Traditional Chinese Medicine,the Third Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650500,China Yunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment,Yunnan University of Chinese Medicine,Kunming 650500,China
医药卫生
postmenopausal osteoporosisnetwork pharmacologymolecular docking simulation
《Traditional Medicine Research》 2026 (10)
P.34-46,13
jointly funded by the National Natural Science Foundation of China(82560949)the joint project of basic research in undergraduate colleges and universities of Yunnan Provincial Department of science and technology(No.202401BA070001-064)the Yunnan Provincial Science and Technology Plan Project-Major Science and Technology Special Project-Biological Seed Industry and Deep Processing of Agricultural Products(No.202102AE090031)the Yunnan Yunling Medical Resources Innovation Research Institute,the target of new R&D institutions in Yunnan Province(No.202304BQ040021).
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