不同枸杞水培条件下不定根发生的转录组比较分析OA

INTRODUCTION:Lycium barbarum is an important economic forest tree species,and its cutting propagation efficiency is closely related to the formation of adventitious roots.However,the molecular mechanism of adventitious root formation is not clear,which restricts the large-scale breeding and efficient utilization of Lycium barbarum. RATIONALE:In order to explore the transcriptome differences in adventitious root formation of L.barbarum,three wolf-berry genotypes with different root-forming abilities were used as experimental materials.A hydroponic experiment was conducted to analyze the transcriptional differences during adventitious root formation. RESULTS:Transcriptome sequencing identified 6 448 differentially expressed genes(DEGs),with the L-vs-H group having the highest number of DEGs at 4 413,including 2 583 upregulated and 1 830 downregulated genes.A total of 281 transcription factors were identified,mainly from the MYB,AP2/ERF,and bHLH families,with distinct expression patterns.GO enrichment analysis revealed that 1 714 DEGs were enriched in 32 GO terms.KEGG enrichment analysis indicated that DEGs were mainly enriched in the phenylpropanoid biosynthesis and plant hormone signal transduction pathways.Among these,MYB19(Lba07g01820)is a core gene in the phenylpropanoid pathway,and TIR1(Lba08g00069)is a core gene in the plant hormone signal transduction pathway.Both genes play crucial roles in adventitious root formation in wolfberry.qRT-PCR validated the reliability of the transcriptome data. CONCLUSION:In this study,the transcriptional regulatory network of adventitious root formation in L.barbarum was analyzed,which provided new insights into the root development mechanism of woody plants,revealed the molecular mechanism of adventitious root formation in L.barbarum,and laid an important theoretical foundation for genetic im-provement and efficient breeding of L.barbarum and other woody plants.