基于UPLC-Q-Orbitrap-MS与网络药理学研究核桃肉炒补骨脂治疗肾阳虚型抑郁症的作用机制OA

Objective Based on ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap-MS)and network pharmacology,the chemical components and potential mechanism of action of"Buguzhi stir-fried with Hetaorou"in the treatment of depression of deficiency of kidney yang were systematically analyzed.Methods The chemical composition of the extract of Buguzhi stir-fried with Hetaorou was identified by UPLC-Q-Orbitrap-MS.The active ingredients and their action targets were screened through network pharmacology methods,and a"component-target-pathway"network was constructed.The biological process and signaling pathways of its treatment of depression were explored through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis.Results A total of 38 chemical components were identified from Buguzhi stir-fried with Hetaorou,such as psoralen,isopsoralen,and bavachinin.Network pharmacology screening identified 30 active components,117 intersection targets,and 20 key pathways,involving neuroactive ligand-receptor interaction,5-hydroxytryptamine synapse,phosphoinositide 3-kinase/protein kinase B signaling pathway,etc.Key active components included neobavaisoflavone,psoralen,isopsoralen,etc.Conclusion Buguzhi stir-fried with Hetaorou may exert therapeutic effects on depression of deficiency of kidney yang through multi-component,multi-target,and multi-pathway synergistic actions,regulating mechanisms such as neurotransmitters,anti-inflammation,antioxidation and neuroprotection.