结合化学计量学对不同种植方式甘草质量的差异评价OA

By investigating the wild-like cultivation of Radix Glycyrrhizae,the differences in characteristic indicators of Radix Glycyrrhizae under different field planting methods were explored,and the quality differences of Radix Glycyrrhizae under different planting methods were comprehensively evaluated,so as to provide technical guidance for the standardized artificial cultivation of Radix Glycyrrhizae.The chemical component analysis and comparison of Radix Glycyrrhizae under different planting methods were conducted using techniques such as microscopic identification(MI),thin-layer chromatography identification(TLC),high-performance liquid chromatography(HPLC)content determination,combined with chemometric methods such as principal component analysis(PCA)and hierarchical cluster analysis(HCA),as well as the similarity evaluation system of traditional Chinese medicine chromatographic fingerprint.MI analysis indicated that there were certain differences in the microscopic characteristics of Radix Glycyrrhizae under different cultivation methods.The crystall fibers and calcium oxalate prisms of Radix Glycyrrhizae planted in the field were significantly larger than those of wild-like cultivation and the blank control.PCA and HCA analysis showed that there were significant differences in the contents of glycyrrhizin and glycyrrhonic acid in Radix Glycyrrhizae under different cultivation methods.TLC and HPLC fingerprint analysis indicated that the accumulation of chemical active components such as glycyrrhizic acid and glycyrrhizin in Radix Glycyrrhizae was in the order of wild-like cultivation>field planting>blank control.Therefore,scientific and reasonable field management techniques can effectively promote the accumulation of chemical active components in Radix Glycyrrhizae,improve the quality of Radix Glycyrrhizae,provide technical guidance for the large-scale and standardized cultivation of Radix Glycyrrhizae,and further enhance the competitiveness of Radix Glycyrrhizae planting industry in the local area,serving local economic development.