西洋参须粗多糖体外免疫活性及抗氧化活性研究OA

To investigate the in vitro biological activity of polysaccharides from Panax quinquefolium L.root hairy,five polysaccharide components(PQLP40,PQLP50,PQLP60,PQLP70,PQLP80)were prepared by using different concentrations of ethanol fractionation precipitation,and in vitro immune enhancement and antioxidant activities were evaluated.The effect of each component on the proliferation of RAW 264.7 macrophages was determined by the MTT assay to assess its immune activity;its antioxidant activity was evaluated by measuring its scavenging ability against DPPH·radicals,hydroxyl radicals,superoxide anion radicals,and ABTS+·cation radicals.In terms of immunological activity,both PQLP40(62.50 µg/mL)and PQLP70(31.25 µg/mL,125.00 µg/mL,500.00 µg/mL)could significantly promote the proliferation of RAW 264.7 cells.Regarding antioxidant activity,polysaccharides from different fractions exhibited different free radical scavenging strengths:PQLP80 had the strongest scavenging activity for DPPH·and·OH radicals,PQLP40 was most effective against·O2-radicals,and PQLP60 showed the highest scavenging activity for ABTS+·.The study indicates that the alcohol precipitation concentration affects the in vitro immunomodulatory and antioxidant activities of Panax quinquefolius polysaccharides,and there are differences in activity among polysaccharides obtained at different alcohol concentrations.Among them,PQLP40 and PQLP80 demonstrate the best immuno-antioxidant activities.