雷公藤甲素减轻心肌细胞缺血再灌注损伤的研究OA

Objective To investigate the effect and mechanism of triptolide(Tri)in alleviating ischemia/reperfusion(I/R)injury in cardiomyocytes.Methods H9c2 rat cardiomyocytes were divided into the I/R group,the I/R+Tri group,and the control group.The myocardial enzyme markers reflecting H9c2cell injury,including creatine kinase(CK),creatine kinase isoenzyme(CK-MB),and lactate dehydrogenase(LDH),as well as the indicators reflecting oxidative stress levels in H9c2 cells,including reactive oxygen species(ROS),malondialdehyde(MDA),and superoxide dismutase(SOD),were detected by ELISA.The expression levels of apoptosis-related genes(Bax,p53,Bcl-2,and Caspase-3)were determined by quantitative real-time polymerase chain reaction(qPCR),and the expression levels of apoptosis-related proteins[Bax,Bcl-2,Caspase-3,and cleaved Caspase-3(c-Caspase-3)]were measured by Western blot analysis.Results The levels of CK and LDH in the I/R group and the I/R+Tri group were higher than those in the control group,and the level of ROS in the I/R group was higher than those in the I/R+Tri group and the control group,with statistically significant differences(all P＜0.01).The relative expression levels of Bax,Caspase-3,c-Caspase-3 and the Bax/Bcl-2 ratio in the I/R group were higher than those in the I/R+Tri group and the control group,while the relative expression level of Bcl-2 in the I/R group was lower than those in the I/R+Tri group and the control group.The relative expression levels of Bax,Caspase-3,c-Caspase-3 and the Bax/Bcl-2 ratio in the I/R+Tri group were lower than those in the control group,and the relative expression level of Bcl-2 in the I/R+Tri group was higher than that in the control group,with statistically significant differences(all P＜0.01).Conclusion Tri can alleviate cardiomyocyte I/R injury by reducing oxidative stress and inhibiting mitochondria-related apoptotic factors.