玉米赖氨酸代谢相关ZmL-PO基因的克隆及功能分析OA
Cloning and Function Analysis of the ZmL-PO Gene Related to Lysine Metabolism in Maize
为探究ZmL-PO基因的功能,阐明玉米籽粒赖氨酸(Lysine,Lys)含量增加的分子调控机制,以玉米B73为材料,通过分子克隆获得ZmL-PO编码区并进行序列测序,结合生物信息学分析预测其蛋白功能,并对玉米B73不同组织和时期基因表达模式进行分析.结果表明,克隆获得的ZmL-PO编码区(CDS)长度为1 245 bp,预测ZmL-P O含415个氨基酸,分子量约为43.94 kD,等电点为5.79.生物信息学分析表明,ZmL-PO属于定位在叶绿体中的亲水性蛋白,其二级结构主要为α螺旋.系统发育分析表明,ZmL-P O蛋白与高粱在进化上种属关系最近.qRT-PCR结果显示,ZmL-PO在授粉后20 d籽粒中表达量最高.
To explore the function of the ZmL-PO gene and to clarify the molecular regulatory mecha-nism of the increase in lysine(Lys)content in maize(Zea mays L.)kernels,using maize B73 as the material,the coding region of ZmL-PO was obtained through molecular cloning and its sequence was sequenced.Combined with bioinformatics analysis,the protein function of ZmL-PO was predicted,and the gene expression patterns in different tissues and at different stages of maize B73 were ana-lyzed.The results showed that the length of the CDS of the cloned ZmL-PO was 1 245 bp,and it was predicted to contain 415 amino acids with a molecular weight of approximately 43.94 kD and an isoe-lectric point of 5.79.Bioinformatics analysis indicated that ZmL-PO belonged to a hydrophilic protein located in the chloroplast,and its secondary structure was mainly α-helix.Systematic phylogenetic analysis showed that the evolutionary relationship between ZmL-POprotein and Sorghum bicolor was the closest.The qRT-PCR results showed that the expression level of ZmL-PO was the highest in the kernels 20 days after pollination.
邓磊;张开武;杨锦越;任洪;王伟
贵州省旱粮研究所,贵阳 550006||农业农村部喀斯特山区玉米生物学与遗传育种重点实验室,贵阳 550006贵州省旱粮研究所,贵阳 550006贵州省旱粮研究所,贵阳 550006贵州省旱粮研究所,贵阳 550006贵州省旱粮研究所,贵阳 550006||农业农村部喀斯特山区玉米生物学与遗传育种重点实验室,贵阳 550006
农业科技
玉米L-甲基哌啶氧化酶生物信息学分析功能分析
Zea maysL-pipecolate oxidasebioinformatics analysisfunction analysis
《种子》 2026 (4)
40-47,8
科研机构创新能力建设项目(黔科合服企[2022]007)贵州旱地粮食作物基因遗传转化平台建设(黔科中引地[2022]4011)国家自然科学基金(32060461)国家玉米产业技术体系(CARS-02-91)
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