基于miRNA测序的甘薯块根miRNA差异表达及其靶基因分析OA

MicroRNA(miRNA)is a type of endogenously encoded non-coding small RNA molecule,which plays a significant role in plant growth and development,hormone signal transduction and secondary metabolism regulation.In this study,using purple-fleshed Ipomoea batatas(PFSP)and white-fleshed Ipomoea batatas(WFSP)as materials,through small RNA high-throughput sequencing technology combined with bioinformatics analysis,the conserved and new miRNAs in the tubers of the 2 varieties at different anthocyanin accumulation periods were systematically identified,their potential target genes were predicted,and functional annotation was carried out.A total of 12 sRNA sequencing data samples were obtained.After quality control,the Clean Reads of each sample were over 10.69 M,and the Q30 value was above 96.80％.After alignment to the Ipomoea batatas reference genome(Ipomoea batatas.Version1),a total of 294 miRNAs were identified,including 9 known miRNAs and 285 newly predicted miRNAs.The length distribution showed that the most miRNAs were concentrated in 21 nt,and the first base at the 5'end preferred uracil(U).Further,using the TargetFinder software to predict the target genes of differentially expressed miRNAs and combined with GO and KEGG enrichment analysis,it was revealed that they were involved in biological processes such as plant hormone signal transduction,secondary metabolism and stress response.