鲜地黄在血热出血模型大鼠体内代谢产物鉴定OA

AIM To identify the in vivo metabolites of fresh Rehmanniae Radix in blood heat and hemorrhage model rats.METHODS Sixteen rats were randomly divided into two groups and given intragastric administration of normal saline and fresh Rehmanniae Radix extract,respectively,after which ultra-high-performance liquid chromatography coupled with linear ion trap-tandem electrostatic field orbital high-resolution mass spectrometry(UHPLC-LTQ-Orbitrap-MS/MS)was adopted in the identification of prototypical components and metabolites in serum,urine and tissues(heart,liver,spleen,lung,kidney,small intestine).RESULTS Total 51 constituents were identified,including 6 prototypical components and 45 metabolites.In the serum,urine,heart,liver,spleen,lung,kidney,small intestine,5,6,1,1,1,1,1,1,1,4 prototypical components and 9,30,8,11,5,4,6,11 metabolites were identified,respectively.Fresh Rehmanniae Radix was metabolized by pathways such as dehydrogenation,reduction,dehydration,methylation and glucuronidation.CONCLUSION Fresh Rehmanniae Radix occurrs phase Ⅰ and phase Ⅱ metabolisms in blood heat and hemorrhage model rats in vivo,the metabolites are widely distributed in various main organs,which can provide basis for the research on pharmacodynamic material basis and action mechanisms of this medicinal material.