HPLC-MS/MS法同时测定参芎益心颗粒中34种成分的含量OA
Simultaneous content determination of thirty-four constituents in Shenxiong Yixin Granules by HPLC-MS/MS
目的 建立HPLC-MS/MS法同时测定参芎益心颗粒中人参皂苷类[人参皂苷Rg1、Re、Rf、Rb1、Ra1、Rc、Rg2、Rh1、(R)-Rg2、Rb2、(R)-Rh1、Rb3、Ro、Rd、Rg3、(R)-Rg3]、酚酸类(丹参素、原儿茶酸、新绿原酸、绿原酸、原儿茶醛、隐绿原酸、咖啡酸、丁香酸、对香豆酸、阿魏酸、迷迭香酸、丹酚酸B)、丹参酮类(二氢丹参酮Ⅰ、丹参酮Ⅰ、隐丹参酮、丹参酮ⅡA)、黄酮类(金丝桃苷、异槲皮苷)的含量.方法 分析采用phenomenex Luna C18色谱柱(150 mm×4.6 mm,5 μm);流动相0.01％甲酸-乙腈,梯度洗脱;体积流量0.4mL/min;柱温30 ℃;电喷雾离子源;正负离子扫描;多反应监测模式.结果 34种成分在各自线性范围内关系良好(r＞0.993 5),平均加样回收率89.33％～118.44％,RSD 1.80％～8.39％.结论 该方法简便可行,稳定可靠,可用于参芎益心颗粒的质量控制.
AIM To establish an HPLC-MS/MS method for the simultaneous content determination of ginsenosides[ginsenosides Rg1,Re,Rf,Rb1,Ra1,Rc,Rg2,Rh1,(R)-Rg2,Rb2,(R)-Rh1,Rb3,Ro,Rd,Rg3,(R)-Rg3],phenolic acids(danshensu,protocatechuic acid,neochlorogenic acid,chlorogenic acid,protocatechuic aldehyde,cryptochlorogenic acid,caffeic acid,syringic acid,4-hydroxycinnamic acid,ferulic acid,rosmarinic acid,salvianolic acid B),tanshinones(dihydrotanshinone Ⅰ,tanshinone Ⅰ,cryptotanshinone,tanshinone Ⅱ A)and flavonoids(hyperoside,isoquercitrin)in Shenxiong Yixin Granules.METHODS The analysis was performed on a 30 ℃ thermostatic phenomenex Luna C18 coclumn(150 mm×4.6 mm,5 μm),with the mobile phase comprising of 0.01％formic acid-acetonitrile flowing at 0.4 mL/min,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Thirty-four constituents showed good linear relationships within their own ranges(r＞0.993 5),whose average recoveries were 89.33％-118.44％with the RSDs of 1.80％-8.39％.CONCLUSION This simple,feasible,stable and reliable method can be used for the quality control of Shenxiong Yixin Granules.
陈秋谷;赖珍沁;吴圆圆;栾杰男;张尚斌;陈剑平
广州中医药大学第四临床医学院深圳市中医院,广东 深圳 518033||深圳市医院中药制剂研究重点实验室,广东 深圳 518033广州中医药大学第四临床医学院深圳市中医院,广东 深圳 518033广州中医药大学第四临床医学院深圳市中医院,广东 深圳 518033广州中医药大学第四临床医学院深圳市中医院,广东 深圳 518033广州中医药大学第四临床医学院深圳市中医院,广东 深圳 518033||深圳市医院中药制剂研究重点实验室,广东 深圳 518033广州中医药大学第四临床医学院深圳市中医院,广东 深圳 518033||深圳市医院中药制剂研究重点实验室,广东 深圳 518033
医药卫生
参芎益心颗粒化学成分含量测定HPLC-MS/MS
Shenxiong Yixin Granuleschemical constituentscontent determinationHPLC-MS/MS
《中成药》 2026 (5)
1429-1435,7
深圳市科技创新委员会项目(JCYJ20220818103402006,ZDSYS201606081515458)深圳市医疗卫生"三名工程"项目(SZZYSM202111002)中医药广东省实验室项目(HQCML-C-2024005)
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