肠道菌群、血浆蛋白与2型糖尿病性骨质疏松症:一项孟德尔随机化研究OA

Objective To investigate the causal relationship between gut microbiota and type 2 diabetic osteopo-rosis(T2DOP)and to evaluate the potential mediating role of plasma proteins in this association.Methods This study employed a two-sample Mendelian randomization(MR)design using summary-level data from genome-wide association studies of gut microbiota(n=8 208),plasma proteins(n=4 719),and T2DOP(1 301 cases and 38 317 controls).The inverse variance weighted(IVW)method was applied as the primary approach to estimate causal effects,comple-mented by multiple sensitivity analyses to assess the robustness and reliability of the findings and the direction of causality.Furthermore,a two-step MR analysis was conducted to evaluate the potential mediating role of plasma proteins in the rela-tionship between gut microbiota and T2DOP.Results Genetically predicted gut microbiota composition showed two posi-tive and three negative causal associations with T2DOP.In addition,21 positive and 23 negative causal associations were identified between plasma proteins and T2DOP.However,no evidence supported a mediating role of plasma proteins in the association between gut microbiota and T2DOP.Conclusion Our findings suggest that both gut microbiota and plasma proteins are causally associated with T2DOP.Nevertheless,plasma proteins do not appear to mediate the effect of gut mi-crobiota on the development of T2DOP.