青霉素V钾可溶性粉对鸡坏死性肠炎的临床疗效研究OA

This study aimed to evaluate the therapeutic efficacy of penicillin V potassium soluble powder against necrotic enteritis(NE)in chickens caused by Clostridium perfringens and to determine an appropriate dosage regimen.A total of 250 chickens naturally infected with NE were randomly divided into five groups:high-dose group(HDG),medium-dose group(MDG),low-dose group(LDG),negative control group(NCG),and positive drug control group(LHS).Chickens in the HDG,MDG,and LDG groups were orally administered penicillin V potassium soluble powder at doses of 102,68,and 46 mg/(kg·bw),respectively.Chickens in the LHS group received lincomycin hydrochloride soluble powder(1.5 g/1 L drinking water),administered once daily for 5 consecutive days.Chickens in the NCG group were given an equivalent volume of physiological saline.Clinical observations were conducted before treatment(D0),on day 5 of treatment(D5),and on days 7(D12)and 14(D19)after treatment.The mental status,feed intake,and diarrhea symptoms of chickens were scored.Body weight,feed intake,and feed conversion ratio were recorded.Cloacal swabs and intestinal tissue samples were collected for isolation and identification of C.perfringens.During the experiment,dead chickens were necropsied and subjected to pathological examination.At the end of the trial,treatment efficacy was evaluated.The results showed that chickens in all groups exhibited varying degrees of depression,reduced feed intake,and diarrhea.Compared with the NCG group,the LDG,MDG,HDG,and LHS groups showed significantly lower scores for mental status,feeding behavior,and diarrhea symptoms at D5,D12,and D19(P<0.05),and significantly increased body weight(P<0.05).Feed intake during D0-D19 was significantly increased in the treatment groups compared with the NCG group(P<0.05).The highest feed conversion ratio during D1-D5 was observed in the NCG group(4.73).The detection rate of C.perfringens in the LDG,MDG,HDG,and LHS groups at D5,D12,and D19 was markedly lower than that in the NCG group.During the experiment,7 chickens died in the NCG group,6 in the LDG group,and 1 in the HDG group.Necropsy of the dead chickens revealed typical lesions of NE.The overall cure rates in the LDG,MDG,HDG,and LHS groups were 62%,86%,86%,and 86%,respectively.These results indicate that penicillin V potassium soluble powder at a dose of 68 mg/(kg·bw)is an effective clinical treatment for NE caused by C.perfringens.The findings provide treatment evidence to support its clinical application.