牛血浆中莫昔克丁和三氯苯达唑LC-MS/MS检测方法的建立与验证OA

To simultaneously determine moxidectin(MOX),triclabendazole(TCB),and its metabolites triclabendazole sulphoxide(TCB-SO)and triclabendazole sulphone(TCB-SO₂)in bovine plasma,an ultra-performance liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was developed and validated for specificity,linearity,limit of detection(LOD),limit of quantification(LOQ),matrix effect,recovery,precision,dilution effect,and stability.The results showed that the developed method exhibited good specificity for the simultaneous determination of MOX,TCB,and their metabolites in bovine plasma.MOX(0.25-20.00 ng/mL),TCB(5-500 ng/mL),TCB-SO(50-5 000 ng/mL),and TCB-SO₂(50-5 000 ng/mL)showed good linearity within their respective ranges,with correlation coefficients(R²)all exceeding 0.990 000.The LODs of MOX,TCB,TCB-SO,and TCB-SO₂ were 0.125,2.5,25,and 25 ng/mL,respectively,and the LOQs were 0.250,5.0,50,and 50 ng/mL,respectively.The coefficient of variation(CV)of the internal standard-normalized matrix factors for low-and high-concentration samples were 0.85%-13.65%and 2.09%-6.26%,respectively,all below 15%.The intra-batch recoveries of the four target compounds in bovine plasma were all above 92%,and the inter-batch recoveries were all above 97%.The intra-batch precision was less than 8%,and the inter-batch precision was less than 7%.For diluted plasma matrix standards,the inter-batch CVs of all four target compounds were below 8.00%.In addition,the method showed good stability,with concentration deviations of less than 15.00%under conditions of long-term freezing,repeated freeze-thaw cycles,room temperature storage,and autosampler storage.These results indicate that the established LC-MS/MS method is stable and reliable and can be used for the simultaneous determination of MOX,TCB,and their metabolites in bovine plasma.