ZBTB20对大鼠肝星状细胞增殖、迁移的影响OA

Objective This study explored the effects of zinc finger and BTB domain protein 20(ZBTB20)on rat hepatic stellate cell(HSC-T6)proliferation and migration,providing experimental evidence for hepatic fibrosis(HF)treatment.Methods ZBTB20 expression in liver tissues of normal person and HF patients were analyzed by the GEO database.Ten SD rats were randomly divided into Sham operation and common bile duct ligation(BDL)groups to establish a cholestatic HF model.Western blot assay was used to detect the expression levels of ZBTB20 in rat liver tissues.Western blot assay was used to detect the expression levels of ZBTB20 and fibrosis markers in HSC-T6 cells after 48 hours of transforming growth factor TGF-β1 stimulation.RT-qPCR and Western blot assays were used to detect the expression levels of the transfection efficiency of ZBTB20 small interfering RNA(si-ZBTB20)and overexpression plasmid(pcDNA3.1-ZBTB20),as well as the expression levels of fibrosis markers in HSC-T6 cells in the si-ZBTB20 and pcDNA3.1-ZBTB20 groups.The effects of si-ZBTB20/pcDNA3.1-ZBTB20 on HSC-T6 cells proliferation,colony formation,and migration were detected through CCK-8,colony formation,and transwell assays.Results ZBTB20 was significantly highly expressed in the liver tissues of HF patients.ZBTB20 expression levels were significantly up-regulated in the BDL group compared with the Sham group(P＜0.05).α-SMA,Col1 A1,and ZBTB20 proteins were significantly up-regulated in HSC-T6 cells in the TGF-β1 group compared with the control group(P＜0.05).RT-qPCR and Western blot assay showed that ZBTB20,α-SMA,Col1A1,and CTGF mRNA expression was down-regulated in the si-ZBTB20-transfected group compared with the si-NC-transfected group,and ZBTB20,α-SMA,and Col1A1 protein expression was decreased(P＜0.05);the above indicators in the pcDNA3.1-ZBTB20-transfected group showed opposite trends compared with the pcDNA3.1-transfected group.CCK-8,colony formation,and Transwell assays showed that cell proliferation in the si-ZBTB20-transfected group decreased significantly after 72 and 96 hours of transfection,the number of cell colonies decreased significantly,and the number of cells passing through the basement membrane of the Transwell chamber decreased significantly compared with the si-NC-transfected group(P＜0.05);the above indicators in the pcDNA3.1-ZBTB20-transfected group showed opposite trends compared with the pcDNA3.1-transfected group.Conclusions ZBTB20 was significantly highly expressed in the liver tissues of patients with HF,and its expression in BDL rat liver tissues increased.ZBTB20 knockdown inhibited HSC-T6 cells proliferation and migration,while its overexpression promoted proliferation and migration.