复合因素诱导轻至中度慢性萎缩性胃炎模型的建立与验证OA

Objective To establish a rat model of mild-to-moderate chronic atrophic gastritis(CAG)induced by multiple factors and to evaluate and validate its application in pharmacodynamic testing.Methods A rat CAG model was established by inducing three composite factors:1-methyl-3-nitro-1-nitrosoguanidine(MNNG)combined with ranitidine and feeding disorders.From 14 to 24 weeks post-modeling,three rats each from the blank and model groups were dissected at each time point to conduct gastric tissue pathological examination to assess model progression.At the mature node of model creation,20 normal rats were selected for the control group,and 40 model rats were randomly and evenly divided into model and folic acid groups.The animals in each group were administered by gavage once daily for 28 d.Body mass and food intake in each group were monitored,and half of the animals in each group were euthanized for gastric tissue pathology examination on days 14 and 28 of treatment.Results Compared with the blank group,model rats showed a significant decrease in body mass starting from day 35 of modeling,and a significant decrease in food intake starting from day 46.After 23 weeks,there was mild to moderate degeneration/necrosis of the mucosal glands in the gastric body and antrum,slight loss of glandular epithelial cells,and mild infiltration of inflammatory cells in the gastric body,which remained stable by week 24.Compared with the model group,the folic acid group showed a significant increase in body mass starting from day 18 of administration,and a significant increase in food intake starting from day 15;gastric atrophy and gastric injury scores were significantly reduced after 28 d of administration,as was the incidence and degree of gastric proper gland atrophy.Conclusions The three-compound factor modeling method induced a stable mild-to-moderate rat CAG model in 24 weeks,which is suitable for drug efficacy evaluation.