基于"以方测证"的寒湿泄泻大鼠模型建立与验证OA

Objective This study is based on the"diagnosis syndrome by prescription"approach and adopts a combination of internal and external factors to construct a rat model of cold-damp diarrhea.Methods 48 rats were randomly divided into a blank control(CON,n=12)group,a cold-damp diarrhea model(CDS,n=12)group,a Lizhong decoction intervention(LCD,n=12)group,and a Pulsatilla decoction intervention(PCD,n=12)group.The model construction used a composite factor:internal factors(psyllium seed combined with ice water gavage)and external factors(cold-damp environmental stimulation)were combined to replicate the rat model of cold-damp diarrhea.The Lizhong decoction and Pulsatilla decoction interventions were carried out for the analysis of the diagnosis syndrome by prescription,and the clinical manifestations of each group of rats were recorded.The atpase activity and the levels of serum interleukin(IL)-6,IL-10,tumor necrosis factors(TNF)-α,and IL-1β were measured in all groups.Results Compared with normal rats,rats in the model group exhibited increased rates of loose stools,higher fecal water content,and elevated diarrhea indices,consistent with the diagnostic criteria for primary and secondary symptoms associated with cold-dampness diarrhea.Notably,serum Ca2+-Mg2+ATPase and Na+-K+ATPase activities were significantly decreased in rats in the model group,while IL-6,IL-1β,and TNF-αlevels were markedly increased and IL-10 levels declined.Prescription testing indicated that Lizhong Decoction had a significant impact on these parameters among rats suffering from cold-dampness diarrhea,while Pulsatilla decoction had no notable effects.Conclusion An effective animal model for cold-dampness diarrhea was successfully established by a"combination of internal causes+external causes",providing a robust methodological foundation for further investigations into the mechanisms underlying diarrhea-related diseases.