"One Health"监测中IncP6型质粒基因组分析与全球质粒序列比较OA

This study was aimed at exploring the global dissemination and genomic characteristics of IncP6-type plasmids carry-ing the Klebsiella pneumoniae carbapenemase(KPC)-encoding blaKPC-2 from a One Health perspective.We analyzed global distribu-tion features of IncP6 plasmids by using the Plasmids in Pathogens Database(PIPdb).Human-,animal-,environmental-,and food-derived samples were collected from Henan Province,China,under the One Health framework.We subsequently screened for carbapenem-resistant strains,performed plasmid incompatibility group typing on the resistant strains,and conducted preliminary identification and antimicrobial susceptibility testing on the screened carbapenem-resistant strains bearing IncP6-type plasmids.Se-quences of chromosomal and plasmid genomes were obtained through combined second-and third-generation sequencing technolo-gies.The genetic correlation between the IncP6 plasmid sequences carrying the blaKPC-2 gene and similar plasmid sequences in global public databases was analyzed.IncP6 plasmids demonstrated pandemic spread;were carried by bacteria from 39 species across 17 gen-era;and were detected in animal,human,food,and environmental samples.The blaKPC-2-carrying IncP6 plasmids exhibited broad host adaptability,extensive geographical distribution,and cross-niche persistence.An environmental isolate of Raoultella ornithino-lytica from Henan carried an IncP6 plasmid bearing blaKPC-2.This strain displayed a multidrug-resistant phenotype with multiple resis-tance genes.The identified IncP6 plasmid showed a certain genetic distance from related plasmids in public databases,and carried both the epidemic core resistance structure(ISKpn6/blaKPC-2/ΔblaTEM-1/ISKpn27)and a novel mobile genetic element(IS15DIV/msrE/mphE/IS15DIV).The IncP6 plasmid is widely present globally.The blaKPC-2-carrying IncP6 plasmid demonstrated stability and trans-missibility,and has been transferred across animal-human-environmental compartments.Future monitoring of the molecular charac-teristics and global spread of the blaKPC-2-carrying IncP6 plasmid from a One Health perspective is therefore important.