不同强度静磁场对人角质形成细胞、成纤维细胞及小鼠成纤维细胞生物学特性的影响OA

Objective The study investigates the effects of varying intensities of static magnetic fields on the proliferation and migration capabilities,as well as the cell cycle dynamics,of Normal Human Epidermal Keratinocytes(NHEK),Normal Human Dermal Fibroblast(NHDF),and National Institutes of Health 3T3(NIH-3T3).Methods After culturing human epidermal NHEK,NHDF,and NIH-3T3 adherently,four static magnetic field intervention modes were established.A spatial magnetic field distribution instrument was utilized to assess the relevant indices of various specifications of neodymium iron boron magnets.The morphological changes of the three cell types were observed 72 hours post-intervention with the four static magnetic fields,and their proliferation capabilities were evaluated using CCK8 assays.Flow cytometry was employed to analyze alterations in the cell cycle following 72 hours of static magnetic field intervention across different cell types.Additionally,a wound healing assay was conducted to examine variations in cellular migration abilities after 0、24 and 48 hours of exposure to distinct static magnetic field interventions.Results The four types of magnets used in the study were as follows:N-300 mT pole at 300 mT,S-150 mT pole and N-150 mT pole ranging from 100 to 150 mT,and N-S-100 mT group at 50-100 mT.After a 72-hour intervention with these static magnetic fields,no significant changes were observed in the morphology of three cell types.In NHEK and NHDF,the proliferation ability in the N-300 mT pole group was significantly enhanced compared to the Blank group(P＜0.05).Conversely,the S-150 mT pole group exhibited reduced cell proliferation compared to the Blank group(P＜0.05).In NIH-3T3,an increase in proliferation ability was also noted for the N-300 mT pole group relative to the Blank group(P＜0.05).Conclusion N-pole magnetic field intervention can stimulate the proliferation of NHEK,NHDF and NIH-3T3.S-pole magnetic field intervention can inhibit the proliferation of the three types of cells.The effects of static magnetic field intervention intensity on the cell cycle,proliferation and migration abilities of the three types of cells are different,while magnetic intervention in different directions does not show significant differences.