皮肤镜结合皮肤生理指标检测在3%十二烷基硫酸钠斑贴试验刺激反应中的应用OA
Application of Dermoscopy and Skin Physiological Index to the 3％ Sodium Dodecyl Sulfate in Patch Test Irritation Response
目的:探讨采用皮肤镜、皮肤生理指标测量相结合的结果评价方法,在缩短斑贴试验周期,提高斑贴试验结果判断准确性,提高受试者或患者检测率中的应用价值.方法:在14名健康受试者使用3%十二烷基硫酸钠进行斑贴试验分别敷贴6 h和24 h形成皮肤刺激反应模型,同时以蒸馏水和空白滤纸作为阴性和空白对照,在去除斑贴器后0.5 h、24 h和48 h,分别进行视觉评分、检测皮肤含水量、TEWL、皮肤红斑指数a*值等无创皮肤生理指标,同时使用皮肤镜观察皮肤镜下特征.结果:敷贴6 h组,3%SLS阳性部位共有5例出现Ⅳ级反应、6例出现Ⅱ级、3例出现Ⅰ级反应.敷贴24 h组,3%SLS阳性部位共有14例出现Ⅳ级反应.敷贴6 h组和敷贴24 h组,在去除斑试器后0.5 h,3%SLS阳性对照部位的皮肤含水量明显高于阴性和空白对照部位(P＜0.05),在去除斑试器后48 h,3%SLS阳性对照部位的皮肤含水量明显低于阴性对照部位(P＜0.05).敷贴6 h组和敷贴24 h组,在去除斑试器后0.5 h、24 h和48 h,3%SLS阳性对照部位TEWL值和红斑指数a*值均明显高于阴性对照部位和空白对照部位(P＜0.05).敷贴6 h组,3%SLS阳性对照部位皮肤镜可见在橙黄色背景下斑块状轻度红斑及散在分布的线性血管扩张等刺激反应特征;敷贴24 h组,3%SLS阳性对照部位皮肤镜下可见在粉红色背景下明显红斑、较多线性血管扩张等特征;在去除斑试器24 h和48 h后,皮肤镜下可见在红斑和线性血管基础上出现褐黑色片状痂皮.结论:采用3%SLS敷贴6 h即可形成与敷贴24 h效果一致的阳性皮肤斑贴试验刺激性模型.如果致敏物质为强致敏物质时,人体皮肤斑贴试验敷贴时间可以缩短至6 h,既可以缩短整个斑贴试验时间,也可以减少强致敏物导致的不良反应.
Objective To explore the application value of the result evaluation method combining dermoscopy and skin physiological index measurement in shortening the patch test cycle,improving the accuracy of patch test result judgment,and increasing the detection rate of subjects or patients.Methods Skin irritation response models were formed by applying 3%sodium dodecyl sulfate patches to 14 healthy subjects for 6 hours and 24 hours respectively.Meanwhile,distilled water and blank filter paper were used as negative and blank controls at 0.5 hours,24 hours and 48 hours after removing the patches.Visual scoring was conducted respectively,and non-invasive skin physiological indicators such as skin water content,TEWL,and skin erythema index a* value were detected.Meanwhile,dermoscopy was used to observe the characteristics under dermoscopy.Results In the 6 h group,there were 5 grade Ⅳ reactions,6 grade Ⅱ reactions,and 3 grade Ⅰ reactions in the 3%SLS-positive sites.In the 24 h group,there were 14 grade Ⅳ reactions in the 3%SLS-positive sites.In the 6 h and 24 h groups,skin water content at the 3%SLS-positive control site was significantly higher than that at the negative and blank control sites at 0.5 hours after spot tester removal(P＜0.05),and skin water content at the 3%SLS-positive control site was significantly lower than that at the negative control site at 48 hours after spot tester removal(P＜0.05).In the 6 h and 24 h groups,TEWL and erythema index a* were significantly higher at the 3%SLS positive control site than at the negative control site and blank control site at 0.5,24,and 48 hours after removal of the spot tester(P＜0.05).In the 6 h group,dermoscopy of the 3%SLS-positive control site showed irritant reaction features such as patchy mild erythema and scattered linear vasodilation on an orange background.In the 24 h group,dermoscopy of the 3%SLS-positive control site showed features such as more pronounced erythema,more linear vasodilation,and brownish-black scaling on a pink background.Conclusion Application of 3%SLS for 6 hours resulted in a positive skin patch test irritation model consistent with the effect of 24 hours of application.If the sensitizing substance is a potent sensitizer,the application time of the human skin patch test can be shortened to 6 hours,which may both reduce the total patch test time and reduce the adverse reactions caused by the potent sensitizer.
叶聪秀;易金玲;苏真;林燕雪;赖维
中山大学附属第三医院皮肤与整形美容科 广东 广州 510630中山大学附属第三医院皮肤与整形美容科 广东 广州 510630中山大学附属第三医院皮肤与整形美容科 广东 广州 510630中山大学附属第三医院皮肤与整形美容科 广东 广州 510630中山大学附属第三医院皮肤与整形美容科 广东 广州 510630
医药卫生
皮肤斑贴试验刺激反应十二烷基磺酸钠皮肤镜皮肤角质层含水量经皮水分丢失量(TEWL)红斑指数
skin patch testirritation responsesodium dodecyl sulfatedermoscopycuticle water contenttransdermal water loss(TEWL)erythema index
《中国美容医学》 2026 (6)
1-6,6
国家药品监督管理局化妆品风险评估重点实验室开放性课题(编号:KF2022009)
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