云芝多糖对急性肺损伤小鼠的保护作用及对肠道菌群的影响OA

AIM:To investigate the protective ef-fect of Coriolus versicolor polysaccharide on acute lung injury(ALI)mice and its influence on the in-testinal microbiota.METHODS:Twenty-four mice were randomly divided into three groups:FC group(control group),FM group(LPS induced group),and FY group(Cordyceps polysaccharide prevention group).Mice in the FY group were given 200 mg/kg Coriolus versicolor polysaccharide by gavage daily,while mice in the FC group and FM group were giv-en the same volume of normal saline by gavage dai-ly for 15 days.24 hours later,LPS(13 mg/kg)was in-tratracheally instilled into mice in the FM group and FY group to establish the ALI model.12 hours after the model was established,samples were collected from the mice.The levels of IL-6,IL-10,IL-1β,TNF-α,T-AOC,SOD,GSH-Px,and MDA in the serum of the mice were detected;the pathological morphology changes of the lung tissue were observed via HE staining;and the changes in the intestinal microbio-ta of the mice were analyzed by sequencing tech-nology.RESULTS:Compared with the FM group,the inflammatory cell infiltration in the lung tissue of mice in the FY group was reduced,and the thicken-ing of the alveolar septum,pulmonary edema,and congestion of capillaries were improved;the levels of IL-6,IL-1β,and TNF-α in the blood were signifi-cantly decreased,and the antioxidant capacity was significantly increased.The analysis of the intestinal microbiota revealed that Coriolus versicolor polysaccharide regulated the relative abundance of the intestinal microbiota in mice,increasing the rel-ative abundance of probiotics such as Lactobacillus,Actinomyces,and Bifidobacterium,and reducing the relative abundance of harmful bacteria such as Prevotella,maintaining intestinal homeostasis.CONCLUSION:Coriolus versicolor polysaccharide can exert anti-ALI effects by reducing inflammatory responses,enhancing antioxidant capacity,and reg-ulating the intestinal microbiota of mice.