法半夏通过巨噬细胞和成纤维细胞的Crosstalk减轻COPD损伤机制研究OA

AIM:To explore the mechanism of Processed Pinellia ternata in treating chronic ob-structive pulmonary disease(COPD).METHODS:Forty male C57BL/6J mice were randomly divided into control group(n=8),model group(n=12),Pro-cessed Pinellia ternata group(1.5 g/kg,n=12),and aminophylline group(1 mg/kg,n=8).COPD model was established using smoke inhalation combined with lipopolysaccharide nasal drops.HE staining was employed to observe pathological damage in lung tissue.Lung tissue fibrosis was detected by Masson,MMP2,and MMP9 staining.Fibroblast transformation was tested α-SMA staining.CD206 and F4/80 staining were used to evaluated M2 macrophages polarization.The expression of TGF-β1 and Arg-1 genes were checked though qPCR,the level of Stat1 and p-Stat1 proteins were measured by Western blotting.In addition,macrophages and fibroblasts were co-cultured by transwell chambers,the(cells)were divided into blank control group,normal control group,model group,and low-,medi-um-,high-dose Pinellia ternata group groups(10,20,40 μg/mL,respectively),the fibroblasts transfor-mation was detected by α-SMA staining.RESULTS:Processed Pinellia ternata can significantly alleviate COPD injury(P<0.01),inhibit lung tissue prolifera-tion and fibrosis(P<0.001),reduce the relative ex-pression levels of TGF-β1 and Arg-1 genes(P<0.05),inhibit fibroblast transformation(P<0.05),reduce M2 macrophage polarization(P<0.05)and increase the p-Stat1/Stat1 ratio(P<0.05).CONCLUSION:Pro-cessed Pinellia ternata improves the fibrosis pro-cess in COPD by macrophages and fibroblasts crosstalk blocking,which may be related to sup-pressed M2 macrophage polarization and activated the Stat1 pathway.