益气活络方通过调控JAK2/STAT3通路对类风湿关节炎模型大鼠Th17/Treg平衡的影响OA

AIM:To investigate the therapeutic ef-fect of Yiqi Huoluo Formula on collagen-induced arthritis(CIA)rat models and its influence on the Th17/Treg balance by regulating the JAK2/STAT3 pathway.METHODS:The CIA rat models were es-tablished using bovine type II collagen emulsion in-duction,with tofacitinib citrate serving as the posi-tive control.After 28 days of intervention with dif-ferent doses of Yiqi Huoluo Formula,the degree of joint swelling and the arthritis index scores of the rats were observed.Pathological and morphologi-cal changes in the joints were evaluated using HE staining.The serum levels of IL-17,IL-6,TNF-α,and IL-10 were detected by ELISA.The proportions of Th17 and Treg cells in peripheral blood were mea-sured by flow cytometry.The mRNA expression lev-els of RORγt and Foxp3 in synovial tissues were de-tected by qRT-PCR.The protein expression levels of RORγt,Foxp3,p-JAK2,and p-STAT3 in synovial tis-sues were determined by Western blot.RESULTS:Yiqi Huoluo Formula effectively reduced the degree of joint swelling and the arthritis index scores in CIA rats(P<0.01),and improved the histopathological damage of the joint tissues.It decreased the serum levels of IL-17,IL-6,and TNF-α,as well as the Th17/Treg ratio in peripheral blood,while increas-ing the IL-10 levels(P<0.05,P<0.01).The formula down-regulated the mRNA and protein expression levels of RORγt and up-regulated the mRNA and protein expression levels of Foxp3 in the synovial tissues of rats(P<0.05,P<0.01).In addition,it signif-icantly inhibited the protein expression levels of p-JAK2 and p-STAT3 in synovial tissues(P<0.05,P<0.01).CONCLUSION:Yiqi Huoluo Formula can sig-nificantly alleviate arthritis symptoms in CIA model rats by inhibiting the JAK2/STAT3 signaling pathway and thereby regulating the Th17/Treg balance.