艾司氯胺酮对卵巢癌细胞增殖的影响及机制研究OA

Objective To investigate the effects of esketamine(ESK)on the proliferation of ovarian cancer cells and its mechanism of action.Methods Ovarian cancer SKOV3 cells were cultured in vitro and treated with different concentrations(0.5 mmol/L,1.0 mmol/L,2.0 mmol/L)of ESK,while the control group(0 mmol/L ESK)was established simultaneously.Cell proliferation and colony formation were detected by CCK-8 assay and colony formation assay,respectively.Flow cytometry was used to detect cell apoptosis and cell cycle distribution.Western blot was used to detect the expression levels of Wnt/β-catenin pathway-related proteins(Wnt3a and β-catenin),apoptosis-related proteins[B-cell lymphoma-2(Bcl-2),Caspase-3,and Bcl-2-associated X protein(Bax)],and proliferation-related proteins(Ki67 and cyclin D1).Results Compared with the control group,ESK treatment at different concentrations significantly increased the proliferation inhibition rate of SKOV3 cells,apoptosis rate,percentage of cells in the G0/G1 phase,as well as the expression of Bax and Caspase-3 proteins(P<0.05).Meanwhile,the number of colonies,percentage of cells in the G2/M phase,and the expression levels of Wnt3a,β-catenin,Ki67,cyclin D1,and Bcl-2 proteins were significantly decreased(P<0.05),showing a concentration-dependent effect(P<0.05).Conclusion ESK can inhibit the proliferation of ovarian cancer cells,and its effect may be achieved by regulating the Wnt/β-catenin signaling pathway.