CRISPR/Cas系统在动物疫病诊断中的应用OA

As an emerging molecular diagnostic tool,clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated proteins(Cas)system has been developed into a novel technology for rapid detection of nucleic acids,benefiting from its advantages such as high sensitivity,high specificity,accessibility and suitability for on-site testing.Here,the CRISPR/Cas system was summarized,including its basic principles,immune mechanisms and classification.The characteristics of its three effector proteins,including Cas9,Cas 12 and Cas 13,and their applications in the detection of animal diseases were clarified.An integrated single-or multi-plex amplification and detection system was formed by the integration of different CRISPR/Cas systems with various isothermal nucleic acid amplification techniques,which was described in this review.It was demonstrated that,by numerous studies,the system was highly specific and sensitive,time-saving and free from complex instruments,and had been successfully applied for the simultaneous detection of multiple pathogens,as well as for typing and identification of same pathogen species,thereby demonstrating capability of precise detection.In addition,current challenges and future directions of the CRISPR/Cas system were also discussed.The application of CRISPR/Cas technology in animal disease diagnosis was reviewed,aiming to offer insights and references for future development of rapid,accurate and low-cost detection methods suitable for field application.