16S rRNA基因在3只华南兔残体鉴定上的应用OA

[Objectives]To address the problems of identifying species of wild animal remains involved in legal cases,and to provide standardized technical references for wildlife forensic identification.[Methods]The 16S rRNA gene sequencing technology was used to analyze 3 samples of animal remains from a wild hare traf-ficking case in Shitou Township,Huixian City,Henan Province in December 2023.The species of the samples were identified via DNA extraction,PCR amplification,sequencing,sequence alignment,combined with ana-lyzing genetic distance and constructing phylogenetic trees.[Results]The similarity between the sequences of 16S rRNA gene in the 3 wild hare samples and Lepus sinensis was over 98%,and clustered into one branch in the phylogenetic tree with a support rate of 98%.The species of the 3 samples was clearly identified to be Lepus sinensis.The amount of money involved in the case was calculated to be 240 RMB according to relevant standards.[Conclusions]The 16S rRNA gene sequencing technology was used to accurately and effectively iden-tify species from remains of Lepus sinensis in this study,indicating that this technology is feasible for forensic identification of wildlife remains.