吉林地区牛体表寄生蜱鉴定及携带泰勒虫的分子检测OA

To identify the tick species on grazing cattle and the theileria species they harbor in Jilin Province,this study combined morphological observation.The 18S rRNA gene was used to detect Theileria in ticks.A total of 326 collected ticks were identified as Haemaphysalis longicornis and Ixodes persulcatus morphological and molecular methods,accounting for 70.55%and 29.45%respectively.Detection results showed that T.orientalis and T.sinensis were amplified in Jilin area,with an overall infection rate of 6.44%(21/326).Notably,Shulan City had the highest Theileria infection rate(10.78%).Phylogenetic analysis indicated that T.orientalis in Haemaphysalis longicornis was genetically close to the Gansu isolate(MG799815),while T.sinensis in Ixodes persulcatus had a close relationship with the Henan isolate(KX115427).In conclusion Haemaphysalis longicornis is the dominant tick species parasitizing cattle in Jilin,and the ticks carry T.orientalis and T.sinensis.This study provides essential epidemiological data for the prevention and control the prevention and control of bovine theileriosis in Jilin Province.