绵羊BMP15基因FecXGr突变及其生物信息学分析OA

To investigate the potential impact of the FecXGr mutation in the sheep bone morphogenetic protein 15(BMP15)gene on the function of its encoded protein,this study employed bioinformatics methods to compare the physicochemical properties,secondary/tertiary structures,post-translational modification sites,and cross-species conservation of the protein before and after the mutation.Results indicate that the FecXGr mutation did not alter the BMP15 protein's basicity,hydrophilicity,secretory properties,or three conserved glycosylation sites.It only slightly increased the protein molecular weight to 44.91 kDa and total atom count to 6 347;reduced phosphorylation sites by one;The proportion of α-helices and extended chains in the secondary structure decreased,while the proportion of disordered regions increased.β-folds and the tertiary structure remained stable.Cross-species conservation analysis indicated that sheep BMP15 nucleotide sequences and their encoded protein sequences showed the highest consistency with ruminants(goats,cattle)and the lowest with humans,demonstrating high conservation of this gene in ruminants.This study provides theoretical insights for elucidating the mechanism by which the FecXGr mutation regulates sheep reproductive performance and for molecular breeding.