大黄素通过miR-375/KLF5轴减轻变应性鼻炎炎症OA

This study aimed to investigate the effects of emodin(EM)on inflammation in mice with allergic rhinitis(AR).Serum ovalbumin(OVA)-specific immunoglobulin E(IgE)and histamine levels,inflammatory cell counts,and cytokine levels in nasal lavage fluid(NALF)were measured.Pathological changes in nasal mucosal tissue,along with miR-375 and KLF5 mRNA levels,and KLF5 protein expression were assessed using enzyme-linked immunosorbent assay,hematoxylin-eosin and periodic acid-Schiff staining,quantitative real-time polymerase chain reaction,and protein immunoblotting.The results showed that EM treatment reduced serum OVA-specific IgE and histamine levels,the counts of eosinophils,neutrophils,macrophages,and lymphocytes in NALF,as well as the mass concentrations of IL-4,IL-5,and IL-13,along with miR-375 expression in nasal mucosal tissue.Additionally,the hyperplasia of goblet cells in the nasal mucosa tissue was reduced,the infiltration of inflammatory cells was alleviated,while the expression of KLF5 mRNA and KLF5 protein increased,(P＜0.05).These findings indicate that EM can inhibit the inflammatory response in AR mice by downregulating miR-375 and promoting KLF5 expression.